Thymosin stimulates directional migration of human umbilical vein endothelial cells
نویسندگان
چکیده
Thymosm (Ti) is a 4.9 kDa polypeptide that interacts with G-actin and is thought to be an important mediator in cell proliferation, migration, and differentiation. T4 has been identified as a factor involved in the differentiation of human umbilical vein endothelial cells (HUVECs) cultured on Matrigel. Here we have used various in vitro and in vivo migration assays to demonstrate the role of T134 in endothelial cell migration. Our results demonstrate that T4 acts as a chemoattractant for endothelial cells, stimulating the migration of HUVECs in Boyden chambers fourto sixfold over that observed with media alone. Of the primary cell types tested, only human coronary artery cells responded to T4 treatment, suggesting that the migration activity of T(34 was endothelial cell-specific. Tf4 significantly accelerated the rate of migration into the scratch wounded area of a HUVEC monolayer. T4 treatment also increased the production of matrix metalloproteinases that may degrade the basement membrane during angiogenesis. Additional experiments using subcutaneously implanted Matrigel showed that T34 stimulated cell migration in vivo. These results provide the first direct evidence that T34 has chemoattractive activity and promotes angiogenesis by stimulating the migration of endothelial cells.-Malinda, K. M., Goldstein, A. L., Kleinman, H. K. Thymosin stimulates directional migration of human umbilical vein endothelial cells. FASEBJ.
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